Agarose   琼脂糖   产品描述说明: CAS Number 9012-36-6         Descriptions                                          Analysis Note                                                                               The following is a list of properties associated with our agaroses:                                                 Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.                Gel strength - the force that must be applied to a gel to cause it to fracture.                              Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.  Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.   琼脂糖 Application                                                                            Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Has low ethidium bromide and SYBR Green background staining.                                     琼脂糖 Properties                                                                             total impurities……………………………………………… ...... ..........≤ 1.0% Ash     ≤ 10% water EEO ………………………………………………………………………………0.09-0.13                 transition temp……………………………………………………………..gel point 36 °C (± 1.5 °C)          foreign activity …………………………………………………………….DNase, RNase, NICKase, none detected