Deoxyribonuclease I from bovine pancreas                                                                      

Type IV, lyophilized powder, Protein ≥ 80 %, ≥ 2,000 Kunitz units/mg protein                         

Synonym Deoxyribonucleate 5 ′ -oligonucleotido-hydrolase                                             

DNase I                                                                                 

CAS Number 9003-98-9                                                                        

Descriptions                                                                           

Analysis Note …………………………… Protein determined by biuret.

Application ………………………………. Used for the removal of DNA from protein samples.               

Preparation Note ……………………… Derived from New Zealand-sourced pancreas                         

Unit Definition ………………………… One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.                              

Physical form………………………………Lyophilized powder containing calcium chloride               

Properties                                              

type …………………………………………..Type IV      

form …………………………………………..lyophilized powder                                        

mol wt ………………………………………..mol wt ~3 1 kDa                                         

purified by …………………………………..chromatography    

composition ………………………………..Protein, ≥ 80%                                           

foreign activity …………………………….Chymotrypsin ≤ 0.5%                                         

Protease ≤ 0.05%                                                                         

RNase ≤ 0.02%