Deoxyribonuclease I from bovine pancreas
Type IV, lyophilized powder, Protein ≥ 80 %, ≥ 2,000 Kunitz units/mg protein
Synonym Deoxyribonucleate 5 ′ -oligonucleotido-hydrolase
DNase I
CAS Number 9003-98-9
Descriptions
Analysis Note …………………………… Protein determined by biuret.
Application ………………………………. Used for the removal of DNA from protein samples.
Preparation Note ……………………… Derived from New Zealand-sourced pancreas
Unit Definition ………………………… One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.
Physical form………………………………Lyophilized powder containing calcium chloride
Properties
type …………………………………………..Type IV
form …………………………………………..lyophilized powder
mol wt ………………………………………..mol wt ~3 1 kDa
purified by …………………………………..chromatography
composition ………………………………..Protein, ≥ 80%
foreign activity …………………………….Chymotrypsin ≤ 0.5%
Protease ≤ 0.05%
RNase ≤ 0.02% |