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Pepsin 1:10000 胃蛋白酶1:10000
Pepsin from porcine gastric mucosa
Synonym ： Pepsin A
Pepsin from hog stomach
CAS Number ： 9001-75-6
Descriptions:
Analysis Note: E1%/280=14.7 Protein determined by E1%/280
Application: Used to produce F(ab ′ )2 fragments of antibodies.
Biochem/physiol Actions: Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH>6.
Unit Definition: One unit will produce a ΔA 280 of 0.001 per min at pH2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16ml. Light path = 1cm.)
Properties:
form ………………powder mol wt…………….mol wt 35 kDa
Gene Information ………pig ... LOC396892(396892)
Risk Statements: 36/37/38-42 Safety Statements: 22-24-26-36/37
TEST ：SPECIFICATION APPEARANCE： REPORT RESULT
SOLUBILITY
CLEAR TO SLIGHTLY HAZY COLORLESS SOLUTION AT 1 MG/ML IN 0.01N HCL
PROTEIN BY UV ABSORBANCE ：REPORT RESULT LOSS ON DRYING ：NMT 5%
ENZYMATIC ACTIVITY ： 800 TO 2500 UNITS/MG PROTEIN
UNIT DEFINITION
ONE UNIT WILL PRODUCE A CHANGE IN A280 OF 0.001 PER MINUTE AT PH2.0 AT 37DEGC, MEASURED AS TCA-SOLUBLE PRODUCTS USING HEMOGLOBIN AS SUBSTRATE. (FINAL VOLUME = 16ML. LIGHTPATH=1CM.)
RECOMMENDED RETEST ：2 YEARS |
