Peroxidase(POD) 辣根过氧化物酶
Peroxidase from horseradish
Type VI-A, essentially salt-free, lyophilized powder, ~1000 units/mg solid (using ABTS), 250-330 units/mg solid (using pyrogallol)
Synonym : Donor: hydrogen-peroxide oxidoreductase
Horseradish peroxidase
CAS Number : 9003-99-0 (EC) Number : 1.11.1.7
Properties :
type …………Type VI-A form …………essentially salt-free, lyophilized powder
absorbance ratio …………RZ ~3.0 storage temp. ……………2-8°C
TEST : SPECIFICATION APPEARANCE :RED-BROWN POWDER
SOLUBILITY
CLEAR AMBER TO AMBER-BROWN SOLUTION AT 10 MG PLUS 1 ML OF 0.1 M PHOSPHATE BUFFER, PH 6.0
ENZYMATIC ACTIVITY: APPROX. 1000 UNITS PER MG SOLID (USING ABTS)
UNIT DEFINITION
ONE UNIT WILL OXIDIZE 1 MICROMOLE OF 2,2'-AZINO-BIS(3-ETHYLBENZTHIAZOLINE- 6-SULFONIC ACID) PER MINUTE AT 25 DEG C AT PH 5.0.
ENZYMATIC ACTIVITY :250 TO 330 UNITS/MG SOLID (USING PYROGALLOL)
UNIT DEFINITION
ONE UNIT WILL FORM 1.0 MG OF PURPUROGALLIN FROM PYROGALLOL IN 20 SECONDS AT PH 6.0 AT 20 DEG C.
RZ
APPROX. 3.0. THE RZ IS DEFINED AS THE RATIO OF THE ABSORBANCE AT 403NM TO THE ABSORBANCE AT 275NM. THIS VALUE IS AN EXPRESSION OF THE RATIO OF HEMIN TO PROTEIN CONTENT.
RECOMMENDED RETEST SOP QC-12-006 : 5 YEARS |