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PronaseE 链霉蛋白酶E
Application ：
Total degradation of proteins during the isolation of DNA and RNA; (it is not necessary to let pronase self-digest prior to use). Total hydrolysis of proteins in research and for technical purposes. Tissue dissociation in conjunction with collagenase and trypsin. Production of glycopeptides from purified glycoproteins.
Product Description ：
Inhibitors: Complete Protease Inhibitor Cocktail Tablets
Optimum pH: 6.0 - 7.5, depending on type of protease activity
Specific activity: approx. 7,000 U/g lyophilizate at 40°C with casein as the substrate, pH 7.5. One unit is the enzyme activity that liberates folin-positive amino acids and peptides, corresponding to 1 µmol Tyr in 1 min under assay conditions.
Specificity: Mixture of several nonspecific endo- and exoproteases that generally digest proteins down to single amino acids. Resolves carboxylic acids and alcohols.
Working concentration: 0.5 - 2 mg/ml
Contents ： Lyophilizate, non-sterile
Quality ： Contaminants: ≤ 0.002 U DNase/RNase per mg lyophilizate
Note: The preparation contains approximately 20% calcium acetate. |
