RNase A Ribonuclease A from bovine pancreas Synonym:  Pancreatic Ribonuclease                    Ribonuclease I                    Ribonucleate 3’-pyrimidinooligonucleotidohydrolase                    RNase A CAS Number:  9001-99-4              (EC) Number:  3.1.27.5   Descriptions: Analysis Note:     Protein determined by E1%/280. Application:         RNase protection assays Remove unspecifically bound RNA Analysis of RNA sequences Hydrolyze RNA contained in protein samples Purification of DNA Pancreatic RNase A specifically cleaves at the 3’-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2’-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. Preparation Note: Salt fractionated and chromatographically purified. Properties: Type…………………Type I-A          assay ………………≥60% as RNase A (SDS-PAGE) form …………………powder           mol wt ……………mol wt ~13,700 total impurities ……salt, essentially free foreign activity ……………………protease, essentially free storage temp………………−20°C        Safety Statements: 22-24/25 TEST：SPECIFICATION  APPEARANCE：REPORT RESULT  PROTEIN BY UV ABSORBANCE：REPORT RESULT  ENZYMATIC ACTIVITY： MINIMUM 50 KUNITZ UNITS/MG PROTEIN  ENZYMATIC IMPURITIES：<0.001UNITS PROTEASE/MG SOLID  PURITY BY SDS-PAGE：MINIMUM 60%  RECOMMENDED RETEST：2 YEARS