RNase A
Ribonuclease A from bovine pancreas
Synonym: Pancreatic Ribonuclease
Ribonuclease I
Ribonucleate 3’-pyrimidinooligonucleotidohydrolase
RNase A
CAS Number: 9001-99-4 (EC) Number: 3.1.27.5
Descriptions:
Analysis Note: Protein determined by E1%/280.
Application: RNase protection assays
Remove unspecifically bound RNA
Analysis of RNA sequences
Hydrolyze RNA contained in protein samples
Purification of DNA
Pancreatic RNase A specifically cleaves at the 3’-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2’-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.
Preparation Note: Salt fractionated and chromatographically purified.
Properties:
Type…………………Type I-A assay ………………≥60% as RNase A (SDS-PAGE)
form …………………powder mol wt ……………mol wt ~13,700
total impurities ……salt, essentially free
foreign activity ……………………protease, essentially free
storage temp………………−20°C Safety Statements: 22-24/25
TEST:SPECIFICATION
APPEARANCE:REPORT RESULT
PROTEIN BY UV ABSORBANCE:REPORT RESULT
ENZYMATIC ACTIVITY: MINIMUM 50 KUNITZ UNITS/MG PROTEIN
ENZYMATIC IMPURITIES:<0.001UNITS PROTEASE/MG SOLID
PURITY BY SDS-PAGE:MINIMUM 60%
RECOMMENDED RETEST:2 YEARS |