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RNase A

 

RNase A
Ribonuclease A from bovine pancreas
Synonym:  Pancreatic Ribonuclease
                   Ribonuclease I
                   Ribonucleate 3’-pyrimidinooligonucleotidohydrolase
                   RNase A
CAS Number:  9001-99-4              (EC) Number:  3.1.27.5  
Descriptions:
Analysis Note:     Protein determined by E1%/280.
Application:         RNase protection assays
Remove unspecifically bound RNA
Analysis of RNA sequences
Hydrolyze RNA contained in protein samples
Purification of DNA
Pancreatic RNase A specifically cleaves at the 3’-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2’-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.
Preparation Note: Salt fractionated and chromatographically purified.
Properties:
Type…………………Type I-A          assay ………………≥60% as RNase A (SDS-PAGE)
form …………………powder           mol wt ……………mol wt ~13,700
total impurities ……salt, essentially free
foreign activity ……………………protease, essentially free
storage temp………………−20°C        Safety Statements: 22-24/25
TEST:SPECIFICATION
 APPEARANCE:REPORT RESULT
 PROTEIN BY UV ABSORBANCE:REPORT RESULT
 ENZYMATIC ACTIVITY: MINIMUM 50 KUNITZ UNITS/MG PROTEIN
 ENZYMATIC IMPURITIES:<0.001UNITS PROTEASE/MG SOLID
 PURITY BY SDS-PAGE:MINIMUM 60%
 RECOMMENDED RETEST:2 YEARS
 



 
 

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